Order Information
Catalog# Size Price
P20289 100μl $280
 
Specification
Entrez Gene ID 79923
Aliases NANOG
Clone# 1E6C4
MW 35kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human
Immunogen Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
 
Application
WB 1/500 - 1/2000
IHC N/A
ICC 1/200 - 1/1000
FCM N/A
ELISA Propose dilution 1/10000
Description
NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
 
References
1. Genes Cells. 2006 Sep;11(9):1115-23.
2. Mol Biol Cell. 2007 May;18(5):1543-53.