Order Information
Catalog# Size Price
P20118 100μl $280
 
Specification
Entrez Gene ID 7431
Aliases FLJ36605; VIM
Clone# 9E7E7
MW N/A
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human
Immunogen Purified recombinant fragment of Vimentin expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
 
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC N/A
FCM N/A
ELISA Propose dilution 1/10000
Description
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
 
References
1. Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996)
2. Essa, T.M., et al. J. Egyptian Soc. Parasitol. 26:433-442(1996)
3. Chu, Y.W., et al. Amer.J. Pathol. 148: 63-69(1996)