Human iPS Cell Line (Retroviral): #iPS01

Description

Human iPS (induced pluripotent stem) cell line was derived from human foreskin fibroblasts (HFFs) by retroviral expression of OCT4, SOX2, KLF4, and c-MYC genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers SSEA-4 and Nanog, and demonstrate strong endogenous alkaline phosphatase.
Product Specifications
Product Name Human Induced Pluripotent Stem Cells (Retroviral)
Catalog # iPS01
Size 2x105 cells/vial
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon arrival. This product is stable for 6 months when stored as directed.
Quality Control Human iPS cells were grown in human ES medium supplemented with 10 ng/ml of bFGF. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of SSEA-4 and Nanog, as well as the activity of alkaline phosphatase.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
This protocol can be used for culturing human iPS cells. Human iPS cells were generated by transducing source cells with retroviruses individually encoding the four human transcription factors (Oct4, Sox2, Klf4, and c-Myc) that have been shown to induce the reprogramming of somatic cells to a pluripotent state. The cells were derived using morphological selection criteria and without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-4 and Nanog, and demonstrate a strong endogenous AP activity.