Human iPS Cell Line (Episomal, HFF): #iPS11

Product Description

Footprint-free human iPS (induced pluripotent stem) cell line derived from human foreskin fibroblasts (HFFs) by ectopic expression of OCT4, SOX2, KLF4, and c-MYC genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and Nanog, and demonstrate strong endogenous alkaline phosphatase. High viability, low passage iPS cells have been pre-adapted to serum-free, feeder-free culture conditions.

Immuno Staining


Figure 1. Characterization of iPSCs derived from human dermal fibroblasts using ALSTEM episomal vectors. Bright field image of hiPSC colonies (top left), immunostaining of hiPSC colonies expressing ESC specific markers OCT4, SSEA-3, and TRA-1-81.
 
Product Specifications
Product Name Human iPSC Line, Episomal
Catalog # iPS11
Size 2x105 cells/vial
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control Human iPS cells (cat. no. iPS11) were grown in mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of SSEA-3 and Nanog, as well as the activity of alkaline phosphatase.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
  • Overview
  • Procedure
This protocol can be used for culturing human iPS cells. Footprint-free human iPS cells were generated by transiently introducing episomal plasmids encoding the human transcription factors into human foreskin fibroblasts. The cells were derived using morphological selection criteria and without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of footprint-free human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-4 and Nanog, and demonstrate a strong endogenous AP activity.