Mouse iPS Cell Line: # iPS02M

Product Description

Mouse iPS cell line (induced pluripotent stem cell line) was derived from mouse embryonic fibroblasts (MEFs) by retroviral expression of Oct3/4, Sox2, Klf4 and c-Myc genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard mouse ES cell culture conditions, the morphology of mouse iPSCs are identical to that of mouse ES cells. The cells also express the pluripotency markers SSEA-1 and Nanog, and demonstrate strong endogenous alkaline phosphatase activity. Mouse iPS cells are grown on a feeder layer of mouse embryonic fibroblasts (MEFs) and require the pretreatment of the plate with Gelatin.
 
Product Specifications
Product Name Mouse iPS Cell Line
Catalog # iPS02M
Size 2x105 cells/vial
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control Mouse iPS cells were grown in mouse ES medium supplemented with 103 U/ml LIF. Each lot of mouse iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of SSEA-1 and Nanog, as well as the activity of alkaline phosphatase.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
  • Overview
  • Procedure
This protocol can be used for culturing mouse iPS cells. Human iPS cells were generated by transducing source cells with retroviruses individually encoding the four mouse transcription factors (Oct4, Sox2, Klf4, and c-Myc) that have been shown to induce the reprogramming of mouse embryonic fibroblasts into a pluripotent state. The cells were derived using morphological selection criteria and without the use of fluorescent markers or drug selection. When cultured under standard mouse ES cell culture conditions, the morphology of mouse iPS cells is identical to that of mouse ES cells. The cells also express the pluripotency markers SSEA-1 and Nanog, and demonstrate a strong endogenous AP activity.