Neural Stem Cell Line, Human: #hNSC11

Product Description

Neural stem cells (NSCs) are neural progenitor cells that are capable of differentiating to different kinds of cells in the nervous system when under defined conditions. Taking advantage of their consistent propagation, NSCs have great potential for the study of neurogenesis, neurodegenerative diseases, and clinical transplantation applications. Alstem's human Neural Stem Cell Line is derived from human induced pluripotent stem cells, which are generated from adult skin fibroblasts using Alstem’s unique footprint-free episomal iPSC reprogramming method. These cells are able to proliferate regularly without differentiating to neural lineage cells when cultured in bFGF and EGF containing medium.

Neurons derived from Alstem's NSCs stained with beta-III-tubulin






hNSC Features

  • Derived from footprint-free human iPS cells
  • Homogeneous population
  • Can differentiate to over 90% of neurons under defined conditions

Product Specifications
Product Name Human Neural Stem Cell Line
Catalog # hNSC11
Size 1x106 cells/vial
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control Each lot of hNSCs is tested for growth and viability after recovery from cryopreservation. In addition, each lot is tested for expression of Nestin to ensure its undifferentiating characteristics.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
  • Overview
  • Procedure
This protocol can be used for culturing human neural stem cells. The neural stem cells are derived from ALSTEM's footprint-free iPS cells and each lot is tested for the expression of Nestin to ensure its undifferentiating characteristics.