Human iPS Cell Line (Episomal, CD34+): #iPS16

Product Description

Footprint-free human iPS (induced pluripotent stem) cell line was derived from human bone marrow CD34+ mononuclear cells by ectopic expression of OCT4, SOX2, KLF4, c-MYC and Lin28 genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and Oct4, and demonstrate strong endogenous alkaline phosphatase activity.
 

Figure 1. Characterization of iPSCs derived from human bone marrow CD34+ mononuclear cells. Immunostaining of hiPSC colonies expressing ESC specific markers OCT4, Nanog, and TRA-1-60.
 

Figure 2. The cells have a normal karyotype.
 

Neural rosette
(ectoderm)
Bone
(mesoderm)
Glandular structure
(endoderm)

Figure 3. In vivo pluripotency test by teratoma formation. Various cell types such as neural rosette (ectoderm), bone (mesoderm), and glandular structures (endoderm) are found.
 
Product Specifications
Product Name Human iPSC Line, Episomal (CD34+)
Catalog # iPS16
Size > 2x105 cells/vial
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
  • Overview
  • Procedure
This protocol can be used for culturing human iPS cells. Footprint-free human iPS cells were generated by transiently introducing episomal plasmids encoding the human transcription factors into human peripheral blood mononuclear cells. The cells were derived using morphological selection criteria and without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of footprint-free human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60 and Oct4, and demonstrate a strong endogenous AP activity.