SV40 T Antigen Cell Immortalization Kit: #CILV01

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Description

hTERT Kit
It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.
 
Product Name SV40 T Antigen Cell Immortalization Kit
Catalog # CILV01
Size 5 x 20 µl
Shipping Dry Ice
Storage and Stability Store at -80 °C (stable up to 6 months).
Quality Control The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization kit is functionally tested in fibroblasts.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
Simply infect your cells with our recombinant lentivirus containing SV40 T antigen and screen with puromycin to generate your own immortalized cells.