hTERT Cell Immortalization Kit: #CILV02

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It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. The most recently discovered approach to cell immortalization is through the expression of Telomerase Reverse Transcriptase protein (TERT). It is particularly useful for cells that are most affected by telomere length, including many human cell types. This protein is usually silenced in most somatic cells. These cells are able to avoid replicative senescence by maintaining sufficient telomere lengths when hTERT is exogenously introduced. However, over-expression of hTERT in some cell types (especially in epithelial cells) fails to induce cell immortalization.
Product Name hTERT Cell Immortalization Kit
Catalog # CILV02
Size 5 x 20 µl
Shipping Dry Ice
Storage and Stability Store at -80 °C (stable up to 6 months).
Quality Control The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization kit is functionally tested in fibroblasts.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
  • Overview
  • Procedure
Simply infect your cells with our recombinant lentivirus containing hTERT and screen with puromycin to generate your own immortalized cells.