EZQuant™ Cell Quantifying Kit: #CQ01

(Use coupon code EZQ15 for 15% off 1 x 5ml size)

 

Product Description

The EZQuant™ Cell Quantifying Kit uses WST-8 to conveniently determine the number of viable cells in cell proliferation and cytotoxicity assays. The reagent is reduced by the metabolic activity of live cells and the amount of formazan produced is directly proportional to the number of living cells. One bottle of EZQuant™ yields 500 tests and no premixing is required. Using EZQuant™ for cell proliferation assays has been shown to correlate well with the [3H]-thymidine incorporation assay. In addition, EZQuant™ exhibits a higher detection sensitivity compared to other tetrazolium salts (e.g., MTT, XTT, MTS, or WST-1).

Features

  • High Sensitivity
  • Non-cytotoxic
  • Long Shelf-life
  • Simple Protocol
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Product Specifications
Product Name EZQuant™ Cell Quantifying Kit
Catalog # CQ01
Size 500 tests (CQ01) / 2500 tests (CQ05) / 5000 tests (CQ10)
Shipping Room Temperature
Storage and Stability Store at either room temperature (stable up to 6 months), 0-5 °C (stable up to 1 year) or -20 °C (stable up to 2 years).
For frequent use, store at 0-5 °C. Thawing and freezing repeatedly will cause a background increase that will interfere with the assay.
Quality Control Appearance, blank, and sensitivity tests are performed for each lot. Only lots that pass the following criteria are offered:
Blank Absorbance (460 nm) ≤ 0.700
Sensitivity Absorbance (460 nm) ≥ 1.000
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Cell Count Protocol
  • Cell Proliferation/Cytotoxicity Protocol
  1. Inoculate cell suspension (100 μl/well) in a 96-well plate. Pre-incubate the plate in a humidified incubator (e.g., at 37° C, 5% CO2).
  2. Add 10 μl of the EZQuant™ solution to each well of the plate.
    Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
  3. Incubate the plate for 1 - 4 hours in the incubator.
  4. Measure the absorbance at 450 nm using a microplate reader.
    To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.