EZStem™ Stem Cell Freezing Medium: #M050

Product Description

EZStem™ Stem Cell Freezing Medium was developed to maintain xeno-free and feeder-free conditions during cryopreservation when culturing human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is a ready-to-use solution for cryopreservation of human ES/iPS cells. Human pluripotent stem cells preserved with EZStem™ Stem Cell Freezing Medium results in high cell viability and recovery, and express typical pluripotent markers after thawing. EZStem™ Stem Cell Freezing Medium has been tested on both human ES and iPS cells. Better results were obtained in comparison with both serum-containing freezing media and competing serum-free products, making this an ideal product for cryopreservation of valuable human pluripotent stem cells.
Product Specifications
Product Name EZStem™ Stem Cell Freezing Medium
Catalog # M050
Size 50 ml
Shipping Ambient temperature
Storage and Stability Store at -20 °C. This product is stable up to 6 months when stored as directed.
Quality Control Human ES cells were frozen using EZStem™ Freezing Medium, then recovered, and AP positive colonies were counted. After recovery, cells preserved with EZStem™ Freezing Medium had a minimum of 10% more AP positive colonies than other freezing methods.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
This protocol can be used for the cryopreservation of human embryonic stem (hES) cells cultured with feeder cells or in feeder-free conditions. The procedure describes the cryopreservation of cells cultured in one well of a 6-well plate. Amounts can be scaled up if freezing multiple wells, however, only 1 ml of cell suspension should be aliquotted into each cryogenic vial. Keep EZStem Freezing Medium on ice at all times.