Retrovirus iPSC Reprogramming Kit: #RF101

Product Description

Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc. These hiPSCs share the properties of self-renewal and pluripotency with human ES cells, and can therefore be used as a renewable source for all differentiated cell types of the body. Human iPSCs can be generated from patients of virtually any genetic background. Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. The VSV-G pseudotyped retrovirus has a wide range of targets including both mammalian and non-mammalian cells, and are usually silenced in ES cells. The retrovirus is commonly used in generating iPSCs because of its high reprogramming efficiency. The Human iPS Cell Reprogramming Retrovirus Kit offers such opportunity to generate iPSCs from various tissues and cell types.
Product Specifications
Product Name Retrovirus iPSC Reprogramming Kit
Catalog # RF101
Content

Retrovirus cocktail containing:
human Oct4 retrovirus (cat# RF01O) 2 vials, 20 μl each
human Sox2 retrovirus (cat# RF01S) 2 vials, 20 μl each
human Klf4 retrovirus (cat# RF01K) 2 vials, 20 μl each
human c-Myc retrovirus (cat# RF01M) 2 vials, 20 μl each
GFP retrovirus (cat# RF01G) 1 vial, 20 μl each
TransPlus virus transduction enhancer (cat# V020) 1 vial, 100 μl

Shipping Dry Ice
Storage and Stability Store at -80 °C. This product is stable up to 6 months when stored as directed.
Quality Control Each lot of Human iPS Cell Reprogramming Retrovirus Kit is tested for sterility and successful reprogramming.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure

Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc. These hiPSCs share the properties of self-renewal and pluripotency with human ES cells, and can therefore be used as a renewable source for all differentiated cell types of the body. Human iPSCs can be generated from patients of virtually any genetic background.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. The VSV-G pseudotyped retrovirus has a wide range of targets including both mammalian and non-mammalian cells, and is usually silenced in ES cells. The retrovirus is commonly used in generating iPSCs because of its high reprogramming efficiency. The Human iPS Cell Reprogramming Retrovirus Kit offers such opportunity to generate iPSCs from various tissues and cell types.

Materials you will need:

Human ES medium

DMEM/F12 Invitrogen 10565-042    
Pen/strep Invitrogen 15140-122 1X 1mL
L-Glutamax Invitrogen 35050-061 2 mM 2 mL
nonessential amino acids Invitrogen 11140-050 0.1 mM 2 mL
2-mercaptoethanol Sigma M7522 0.1 mM (1000x) 0.2 mL
knockout serum replacement Invitrogen 10828-028 20% 40 mL
bFGF StemRD bFGF-050 10 ng/mL 0.2 mL
Total 200 mL