ALSTEM's stem cell scientists are experts in stem cell biology, including reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). ALSTEM’s iPSC generation services include expert scientific consultation to plan your reprogramming project, fast turnaround with scheduled milestone status updates to inform you of the progress of your cells, and comprehensive advice in maintaining your reprogrammed iPS cells upon delivery to your laboratory.

Highlights of our iPSC generation service
  • Multiple options of induction: retrovirus, or episomal vectors
  • Footprint free iPS cells with the episomal method
  • Customers are notified with the progress of reprogramming
 

Lentiviral/retroviral vectors are one of the most efficient methods of gene delivery to mammalian cells both in vitro and in vivo. Through years of experience with lentiviral and retroviral vectors, ALSTEM has developed own proprietary packaging systems and efficient protocols for the rapid generation of pseudoviral particles.

ALSTEM offers express lentiviral/retrovirus packaging service to produce high-quality, high-titer virus particles using your viral construct with turnaround time of 2-3 weeks. Save yourself the time and effort of preparing your own virus, and receive ready-to-transduce viral particles. Learn more about our viral packaging services by clicking on the link below.

 

 

The CRISPR/Cas9 system, consisting of a guide RNA (gRNA) and a Cas9 nuclease, allows researchers to create a RNA-guided site-specific DNA cleavage. ALSTEM is then able to use the double strand break created to modify your target gene by inducing a permanent knock-out, conditional knock-out, point mutation, or insertion.

ALSTEM offers complete gene editing services from designing and cloning your gRNA and HR donor plasmid to generating a stable cell line. Save time and effort by having our genome engineering experts develop your CRISPR/Cas9 system. Learn more about our genome editing services by clicking on the link below.

 

 

Senescence of primary cells is a common frustration that scientists encounter, requiring re-establishment of fresh cultures from tissues. However, this problem can be circumvented by immortalizing cells using genetic manipulation. Immortalized cells are easier to culture and maintain, have an extended replicative capacity, and enable one to use the same consistent cells throughout the research project.

ALSTEM scientists have years of experience with cell line immortalization and are able to successfully immortalize essentially any human and mouse cells using different immortalizing methods. We can help save you time and effort. Try our cell immortalization service risk-free.