Custom CRISPR/Cas9 Genome Editing

The CRISPR/Cas9 system, consisting of a guide RNA (gRNA) and a Cas9 nuclease, allows researchers to create a RNA-guided site-specific DNA cleavage. ALSTEM is then able to use the double strand break created to modify your target gene by inducing a permanent knock-out, conditional knock-out, point mutation, or insertion. If you don't see what you're looking for below, please don't hesitate to Contact Us so that we can provide you with your desired service.

ALSTEM offers complete gene editing services from designing and cloning your gRNA and HR donor plasmid to generating a stable cell line. Save time and effort by having our genome engineering experts develop your CRISPR/Cas9 system.

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  • Multiple Modification Options: Permanent knock-out, conditional knock-out, point mutation, insertion
  • Complete Service: We design and clone your gRNA & HR donor plasmid as well as generate a stable cell line for you
  • Verification: successful gene editing analyzed using HRM and Surveyor assay
  • Timeline: 3-5 months depending on project requirements
  • Required Materials: Customer-desired cells, 2 vials of typical storage size, and indication of appropriate growth medium and conditions
  • Deliverables: ALSTEM typically provides 2-6 vials (>2 x 105 cells/vial) of edited clones

Service Requisition Form

Cell Line Gene Modification Service Requisition Form Download

Ordering Information

Catalog # Service Price
CS-GE01 CRISPR/Cas9 Custom Knock-out & Cell Line Generation Service Inquire
CS-GE02 CRISPR/Cas9 Custom Knock-in & Cell Line Generation Service Inquire
CS-GD01 gRNA Construct Design & Cloning Inquire
CS-GD02 HR Donor Vector Design & Cloning Inquire