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SynFire® Glutamatergic Excitatory Neurons: #1001-1

Product Description

Pure ready-to-use induced pluripotent stem cell (iPSC)-derived Glutamatergic induced neurons (iNs) from NeuCyte. SynFire® iNs are generated using a patented procedure for direct reprogramming and exhibit main characteristics of human primary neurons such as expression of typical pan-neuronal markers and complex electrophysiology (e.g., spontaneous/evoked action potentials, synchronized network activity). Neuronal subtype identity of excitatory iN cells has been confirmed by staining and patch clamping.
Highly functional human iPSC-derived neurons are of particular value due to the unavailability of natural sources. SynFire® iNs exhibit mature synaptic network activity. Synchronous bursting phenotypes resembling those in rodent primary cultures appear within three to four weeks. This degree of maturation is ideal for higher order electrophysiological studies. SynFire® iN cells also represent a versatile in vitro cell system for basic research and disease modeling including in vitro gain-of-function and loss-of-function genetic studies1-3. Moreover, these human iN cells can be used for compound screening applications as well as preclinical safety assessment and chemical neurotoxicity.

References

  1. ZHANG, Y. et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron, v. 78, n. 5, p. 785-98, Jun 2013.
  2. PAK, C. et al. Human Neuropsychiatric Disease Modeling using Conditional Deletion Reveals Synaptic Transmission Defects Caused by Heterozygous Mutations in NRXN1. Cell Stem Cell, v. 17, n. 3, p. 316-28, Sep 2015.
  3. YI, F. et al. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science, v. 352, n. 6286, p. aaf2669, May 2016.
 
Product Specifications
Product Name SynFire® Glutamatergic Excitatory Neurons
Catalog # 1001-1
Size >1x106 cells (1001-1) / >5x106 cells (1001-5)
Shipping Dry Ice
Storage and Stability Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control Neuronal subtype identities have been confirmed by staining and patch clamping.
Safety Precaution ALSTEM highly recommends that protective gloves, a lab coat, and a full-face mask always be worn when handling frozen vials. It is important to note that some liquid nitrogen can leak into the vials when submersed in liquid nitrogen. Upon thawing, the liquid nitrogen returns to the gas phase, resulting in excessive pressure within the vial that can cause the vial to explode or expel the cap with dangerous force.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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