Lentiviral Packaging Mix: #VP100

Description

Lentiviral Packaging Mix is a ready-to-use 3rd generation HIV-based lentiviral packaging system in which the plasmids express the elements required for lentiviral production. For you to produce high quality lentiviral particles, all that’s required is a lentiviral expression vector containing your gene of interest. Lentiviral Packaging Mix contains the essential packaging vectors containing the viral structure proteins and the envelope vector to express the Vesicular Stomatitis Virus glycoprotein (VSVG). The lentiviral packaging mix is TAT-independent because it only supports lentiviral expression vector with a chimeric 5' LTR, in which the HIV promoter is replaced with CMV or RSV.

   

 

Product Specifications
Product Name Lentiviral Packaging Mix
Catalog # VP100
Size 200 µl
Shipping Ambient temperature
Storage and Stability Store at -20 °C. This product is stable for 6 months when stored as directed. Freeze-thaw cycles should be minimized by dividing into single-use aliquots. Store aliquots in the -20 °C freezer until ready for use.
Quality Control Each lot of lentiviral packaging mix is functionally tested in transfection assays using human embryonic kidney 293 cells.
Usage
For 100 mm dish of lentiviral packaging, mix 2.5 μg of lentiviral expression vector with 20 μl of lentiviral packaging mix.
For 150 mm dish of lentiviral packaging, mix 5 μg of lentiviral expression vector with 40 μl lentiviral packaging mix.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
The Lentiviral Packaging Mix is an optimized formulation of third generation of packaging plasmids that supply the helper functions as well as structural and replication proteins in trans that are required to produce high-titer lentivirus.