Lentivirus Precipitation Solution: #VC100

Description

Lentivirus Precipitation Solution is a mixture of polymers optimized for the precipitation of lentiviral particles. It provides a simple, fast and highly efficient method for concentrating lentiviral particles. In this simple protocol, you just mix your lentiviral supernatant with the Lentivirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You'll increase your lentivirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation. The Lentivirus Precipitation Solution is a 5x solution.

Features

  • Easy-to-use: simply mix
  • No ultracentrifugation required
  • Easily scale up for large volumes
  • Up to 100 fold concentration increase
  • Cost effective spin protocol for efficient viral concentration
  • Non-toxic: safe for all cell lines, including ES cells
Product Specifications
Product Name Lentivirus Precipitation Solution
Catalog # VC100
Size 100 ml / 250 ml / 500 ml
Shipping Ambient temperature
Storage and Stability Store at 4 °C. This product is stable for 6 months when stored as directed
Quality Control Each lot of Lentivirus Precipitation Solution is tested for sterility and successful precipitation of lentiviral particles.
Safety Precautions Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
Lentivirus Precipitation Solution is a mixture of polymer optimized for the precipitation of lentiviral particles. It provides a simple, fast and highly efficient method for concentrating lentiviral particles. In the simple protocol, you just mix your lentiviral supernatant with the Lentivirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You’ll increase your lentivirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation.The Lentivirus Precipitation Solution is a 5x solution.