Retroviral Packaging Mix: #VP200

Description

The Retroviral Packaging Mix provides a rapid and convenient method for producing high titers of replication-incompetent retroviruses. This Moloney Murine Leukemia Virus (MMLV)-based packaging system possesses the ideal host range required for your target cells. It is a ready-to-use retroviral packaging mix, which contains VSVG and produces high titer retroviruses in only 48 hours. Separate expression of these essential viral structural genes ensures safety and control over the recombinant constructs by minimizing the risks that arise during cell division and reducing the chance of producing replication-competent viruses.
    
 
Product Specifications
Product Name Retrovirus Packaging Mix
Catalog # VP200
Size 200 µl
Shipping Ambient temperature
Storage and Stability Store at -20 °C. This product is stable for 6 months when stored as directed. Freeze-thaw cycles should be minimized by dividing into single-use aliquots. Store aliquots in the -20 °C freezer until ready for use.
Quality Control Each lot of retroviral packaging mix is functionally tested in transfection assays using human embryonic kidney 293 cells.
Safety Precautions Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Usage For 10 cm dish of retrovirus packaging, mix 7.5 μg of retroviral expression vector with 15 μl of retroviral packaging mix
Protocol Download
 
  • Overview
  • Procedure
The Retroviral Packaging Mix is an optimized formulation for producing high-titer pantropic retrovirus.
  • Achieve average titer of 107 infectious units/ml with transient transfection
  • Simple cotransfection of the retroviral packaging mix and the expression vector containing your gene of interest
  • Produce self-inactive, pantropic retrovirus