Retrovirus Precipitation Solution: #VC200

Description

Retrovirus Precipitation Solution is a mixture of polymers optimized for the precipitation of retroviral particles. It provides a simple, fast and highly efficient method for concentrating retroviral particles. In this simple protocol, you just mix your retroviral supernatant with the Retrovirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You'll increase your retrovirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation. The Retrovirus Precipitation Solution is a 5x solution.

Features

  • Easy-to-use: simply mix
  • No ultracentrifugation required
  • Easily scale up for large volumes
  • Up to 100 fold concentration increase
  • Cost effective spin protocol for efficient viral concentration
  • Non-toxic: safe for all cell lines, including ES cells
Product Specifications
Product Name Retrovirus Precipitation Solution
Catalog # VC200
Size 100 ml / 250 ml / 500 ml
Shipping Ambient temperature
Storage and Stability Store at 4 °C. This product is stable for 6 months when stored as directed
Quality Control Each lot of Retrovirus Precipitation Solution is tested for sterility and successful precipitation of retroviral particles.
Safety Precautions Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
Retrovirus Precipitation Solution is a mixture of polymer optimized for the precipitation of retroviral particles. It provides a simple, fast and highly efficient method for concentrating retroviral particles. In the simple protocol, you just mix your retroviral supernatant with the Retrovirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You’ll increase your retrovirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation.The Retrovirus Precipitation Solution is a 5x solution.