ViralBoost Reagent: #VB100

Description

ViralBoost Reagent (500X) is a novel cocktail of small molecules that can enhance viral production and is a powerful, broadly applicable reagent for effective virus packaging. The ViralBoost Reagent stably regulates the viral RNA packaging on the transcriptional level, which can enhance production of either retro- or lenti-viral particles up to 10 fold. The easy-to-use protocol makes ViralBoost Reagent well-suited for various scales of virus packaging.
 
Fig 1.Flow cytometry data from HEK 293T cells transduced by GFP retroviruses which were packaged in the absence and presence of the ViralBoost Reagent.
Viral Boost Viral Production
 
 
Fig 2.HEK 293T cells transduced by GFP lentivirus which were packaged with and without ViralBoost
ViralBoost Virus Production Increase
 
 
Product Name ViralBoost
Catalog # VB100
Size 1 ml
Shipping Ambient temperature
Storage and Stability Store at 4 °C. This product is stable for 12 months when stored as directed.
Quality Control Each lot of ViralBoost Reagent is functionally tested in transfection assays using human embryonic kidney 293T cells.
Usage 6-14 hours after transfection of human embryonic kidney (HEK) 293T cells with retroviral or lentiviral packaging plasmid mix, replace the culture medium with fresh DMEM medium supplemented with 10% heat-inactivated fetal bovine serum and 0.5% penicillin-streptomycin, and add 1/500 volume of ViralBoost Reagent to one volume of fresh culture medium and continue incubation in the CO2 incubator at 37°C.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Protocol Download
 
  • Overview
  • Procedure
ViralBoost Reagent is a novel cocktail of small molecules that is used for high titer virus production and is a powerful, broadly applicable reagent for effective virus packaging. It stably regulates the viral RNA packaging on the transcriptional level, which can greatly enhance production of either retro- or lenti-viral particles up to 10 fold. The easy-to-use protocol makes ViralBoost Reagent well-suited for various scales of virus packaging.