Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. Lentiviral vectors packaged as lentiviral particles is one of the most efficient tools to deliver exogenous genes into virtually any types of mammalian cells both in vitro and in vivo. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells, with low immune response and toxicity in vivo. These viruses also integrate stably into the host genome, enabling long-term transgene expression. Our 3rd generation lentiviral systems have been designed for increased researcher safety.
pLenti-CAG-GFP-PGK-Neo Lentiviral Reporter Plasmid contains GFP reporter driven by CAG promoter and Neomycin resistance gene is driven by PGK promoter respectively. This reporter vector serves a positive control. The cells transduced by this vector should display green fluorescence.