Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo.
Lentivirus particles are produced from 293T cells through transient transfection of plasmids that encode for the components of the virion. Our third generation lentiviral systems have been designed for increased researcher safety.
pLenti-CMV-GFP-EF1α-RFP-T2A-PURO Lentiviral Reporter Plasmid contains CMV promoter ahead of copGFP with tdTomato reporter and puromycin resistant gene driven by EF1α promoter. This dual reporter vector serves a positive control. The cells transduced by this vector should display both green and red fluoresence.