ABCA7 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)
|Catalog#||Unit||Unit Price (USD)||Actions|
|iPS21||5x105 cells/vial||$2,835.00||Add to Cart|
ALSTEM offers human iPS cell line (iPS21) with ATP-binding cassette sub-family A member 7 (ABCA7) deficiency This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS11) by using gene editing method to introduce a deletion and frame shift in the ABCA7 gene. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 82 bp deletion in the exon 8 of ABCA7 gene. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells.
- Good model for studying AD-related pathologies in neurons induced specifically by ABCA7
- Ideal for drug discovery when used with ABCA7 wild type iPS cell line (cat# iPS11)
ABCA7 protein is a member of the superfamily of ATP-binding cassette (ABC) transporters which regulate the lipid metabolism in the central nervous system, as well as peripheral tissues. Genome-wide association studies (GWAS) identified several ABCA7 variants as novel risk factors for Alzheimer’s disease (AD). In vitro studies indicate over-expression of ABCA7 results in a significant decrease in amyloid-β (Aβ) production, while a more rapid uptake of amyloid precursor protein (APP) in ABCA7 knockout cells is mechanistically consistent with an increased Aβ production. It was therefore suggested that ABCA7 protein directly impacts amyloid-β pathology by altering APP trafficking and substrate availability.
Alstem’s footprint-free human iPS (induced pluripotent stem) cell line (cat# iPS21) carries ABCA7 KO mutation in both alleles. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 82 bp deletion in the exon 8 of ABCA7 gene. The morphology of this human iPS cell line is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and OCT4, and demonstrate strong endogenous alkaline phosphatase activity.
|Product Name||ABCA7 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)|
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|Storage and Stability||
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.