LRP1 Knockout Human Induced Pluripotent Stem Cells (Episomal, HFF)
|Catalog#||Unit||Unit Price (USD)||Actions|
|iPS31||5x105 cells/vial||$2,835.00||Add to Cart|
ALSTEM offers human iPS cell line (iPS31) with low-density lipoprotein (LDL) receptor-related protein (LRP1) deficiency. This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS11) by using gene editing method to introduce a deletion and a frame shift in the LRP1 gene. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 191 bp deletion in the exon 6 of LRP1 gene. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells.
- Good model for studying AD-related pathologies in neurons induced specifically by LRP
- Ideal for drug discovery when used with LRP wild type iPS cell line (cat# iPS11)
LRP1 belongs to cell surface LDL receptor family sharing structural and functional similarity. LRP1 mediates clearance of Aβ from the brain by cellular uptake and consequent lysosomal degradation by various cell types. LRP1-related Aβ clearance mechanisms include intracellular degradation, efflux across BBB to the circulation, and peripheral clearance. Several clinical and preclinical studies indicated that LRP1 is associated with pathogenesis of Alzheimer’s disease (AD). AD is a severe neurodegenerative disorder that deteriorates a person’s cognitive abilities and afflicts an increasing number of our elderly population. AD patients have observed neuronal degeneration and the extracellular deposition of hydrophobic peptide termed β-amyloid (Aβ). LRP1 is suggested to mediate the removal of the Aβ aggregates by directly binding to it and mediating its cellular uptake and degradation.
ALSTEM’s footprint-free human iPS cell line (cat# iPS31) carries LRP1 KO mutation in both alleles. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 191 bp deletion in the exon 6 of LRP1 gene. The morphology of this human iPS cell line is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and OCT4. The cells also demonstrate a strong endogenous alkaline phosphatase activity.
|Product Name||LRP1 Knockout Human Induced Pluripotent Stem Cells (Episomal, HFF)|
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|Storage and Stability||
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.