pLV-EF1-dCas9-KRAB-T2A-PURO
Catalog# | Unit | Unit Price (USD) | Actions |
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DK112 | 10 μg at 0.5 μg/μL in TE | $695.00 | Add to Cart |
Description:
CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi) is a widely used genetic technique that allows targeted silencing of transcription in bacteria and mammalian cells. The CRISPRi system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional repressor such as the Krüppel-associated box (KRAB) repressor and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and causes a steric block that halts transcript elongation by RNA polymerase, resulting in the repression of the target gene.
pLV-EF1-dCas9-KRAB-T2A-PURO Lentiviral Vector expresses the dCas9-KRAB fusion with EF1 promoter and allows for PURO selection of transduced cells.
Vector Information
This is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. A ubiquitous EF1 promoter drives the expression of the dCas9-KRAB fusion protein and PURO to allow for selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specifc sgRNA to repress the target gene expression.
IMPORTANT NOTICE
Store the vial at -20°C immediately upon receipt.
Specifications:
Product Name | pLV-EF1-dCas9-KRAB-T2A-PURO |
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Shipping Condition |
Room Temperature - 2 Day Shipping |
Storage and Stability |
Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed. |
Quality Control | This plasmid is sequence verified. |
Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |