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pLV-EF1-dCas9-KRAB-T2A-PURO

Catalog# Unit Unit Price (USD) Actions
DK112 10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
Description:

CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi) is a widely used genetic technique that allows targeted silencing of transcription in bacteria and mammalian cells. The CRISPRi system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional repressor such as the Krüppel-associated box (KRAB) repressor and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and causes a steric block that halts transcript elongation by RNA polymerase, resulting in the repression of the target gene.

pLV-EF1-dCas9-KRAB-T2A-PURO Lentiviral Vector expresses the dCas9-KRAB fusion with EF1 promoter and allows for PURO selection of transduced cells.

Vector Information

This is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. A ubiquitous EF1 promoter drives the expression of the dCas9-KRAB fusion protein and PURO to allow for selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specifc sgRNA to repress the target gene expression.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

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Note: Bacterial culture of pLenti vectors should be done in medium containing 50 μg/mL Ampicillin. For maximal plasmid yield and quality, we recommend Stbl3 competent cells (Invitrogen).
Specifications:
Product Name pLV-EF1-dCas9-KRAB-T2A-PURO
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

This plasmid is sequence verified.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.