Description:

The Cre/LoxP recombination system is a versatile and powerful tool for DNA manipulations such as deletions, insertions, translocations, and inversions at specific sites in the DNA of cells.  The system uses Cre recombinase to recombine a pair of Lox sites.  Depending on the direction and distance of the LoxP sites, DNA flanked by the LoxP sites can be deleted, inversed, or translocated.

The Cre-GFP Lentivirus Vector (pLenti-EF1-Cre-GFP-PGK-PURO) expresses the Cre-GFP fusion protein in the nucleus and provides puromycin resistance to the cell receiving it. This lentivirus vector can be used for transient Cre expression and for stable cell line development. Robust Cre recombinase activity has been detected in stable transgenic cell lines that carry two loxP sites in the same direction in their transgenes. DNA deletion events were detected by PCR and validated by DNA sequencing.

Vector Information

This is a lentiviral expression vector containing all elements for efficient and high yield viral production. A ubiquitous EF1 drives the expression of the Cre-GFP fusion protein in the nucleus and PURO to allow for selection of transduced cells. This vector can be used for transient Cre expression and stable cell line generation.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

Specifications:

Product Name	pLenti-EF1-Cre-GFP-PGK-PURO
Shipping Condition	Room Temperature - 2 Day Shipping
Storage and Stability	Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control	This plasmid is sequence verified.
Restricted Use	For Research Use Only. Not for use in diagnostic or therapeutic procedures.