Mouse iPS Cell Line
|Catalog#||Unit||Unit Price (USD)||Actions|
|iPS02M||5x10^5 cells/vial||$950||Add to Cart|
iPS02M cell lines generated by retroviral transduction are ideally suited for various research purpose including 1) differentiating various somatic cells or organoid models for phenotypic and target-based compound screening, 2) establishing genetically modified disease model through CRISPR/Cas. iPS02M has several features such as:
- Low passage and long-term viability
- Off the shelf - simple thaw the cells and plate them onto serum-free, feeder-free culture
- Homogeneity– Originated from a single iPSC clone
Mouse iPS cell line (induced pluripotent stem) cell line (Cat# iPS02M) was derived from mouse embryonic fibroblasts (MEFs) by retroviral expression of Oct3/4, Sox2, Klf4 and c-Myc genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard mouse ES cell culture conditions, the morphology of mouse iPSCs are identical to that of mouse ES cells. The cells also express the pluripotency markers SSEA-1 and Nanog, and demonstrate strong endogenous alkaline phosphatase activity. Mouse iPS cells are grown on a feeder layer of mouse embryonic fibroblasts (MEFs) and require the pretreatment of the plate with Gelatin.
|Product Name||Mouse iPS Cell Line|
|Shipping Condition||Dry Ice - Overnight Shipping|
|Storage and Stability||
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Mouse iPS cells were grown in mouse ES medium supplemented with 103 U/ml LIF. Each lot of mouse iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of SSEA-1 and Nanog, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Receptor interacting protein kinase 3 (RIP3) regulates iPSCs generationthrough modulating cell cycle progression genes Stem Cell Research (2019)