hTERT Cell Immortalization Kit
It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. The most recently discovered approach to cell immortalization is through the expression of Telomerase Reverse Transcriptase protein (TERT). It is particularly useful for cells that are most affected by telomere length, including many human cell types. This protein is usually silenced in most somatic cells. These cells can avoid replicative senescence by maintaining sufficient telomere lengths when hTERT is exogenously introduced. However, over-expression of hTERT in some cell types (especially in epithelial cells) fails to induce cell immortalization.
|Product Name||hTERT Cell Immortalization Kit|
Dry Ice - Overnight Shipping
|Storage and Stability||
Store at -80 °C (stable up to 6 months)
The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization kit is functionally tested in fibroblasts.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Benischke AS et al., Sci Rep. 2017 Jul 27;7(1):6656. doi: 10.1038/s41598-017-06523-2.