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Nanoluciferase/tdTomato Dual-Reporter HCT116 Cell Line

Catalog# Unit Unit Price (USD) Actions
NRL02 1 million cells/vial $1575 Add to Cart
Description:

NanoLuc luciferase is a small (19 kDa), highly stable, ATP independent, bioluminescent protein. Compared to firefly luciferase, it is an extremely robust and ultra high sensitivity screening system. It has been widely used as convenient and powerful reporting tools in numerous biomedical researches, such as, cell tagging, protein-protein interaction assays, real-time monitoring and high-throughput drug screening. Nanoluciferase/tdTomato dual-reporter HCT116 (HCT116-NanoLuc-RFP) cell line is derived from HCT116 cells by transduction of ALSTEM’s pLenti-CAG-Nanoluciferase-PGK-tdTomator-T2A-PURO Lentiviral Reporter (cat# LV311L) lentivirus, which contains Firefly Nanoluciferase encoding gene driven by CAG promoter, followed by tdTomato and puromycin resistance gene by PGK promoter. This cell line stably expresses Nanoluciferase at high level. It also expresses a red fluorescent protein (tdTomato) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.

Cell Line: HCT116

Reporters: NanoLuc, RFP

Selection Marker: Puromycin

Promoter/Transgene: CAG-NanoLuc-PGK-TdTomato-T2A-puro

Cell Type: Single Clone

images/Desc_Images/NRL02_1.png
Figure 1. Panel A: RFP Fluorescence. Dual-labeled HCT116 cell line expressing tdTomato at 80% cell confluence. Panel B: Nanoluciferase activity. Comparison of luminescence signal for NanoLuc luciferase and negative control demonstrating its robustness and high sensitivity in the Nanoluciferase/tdTomato dual-labeled HCT116 cells. The Nanoluciferase activity was tested 6 hrs after seeding in a 96-well plate. Data are expressed as mean /- SD from duplicates of three independent experiments.
images/Desc_Images/NRL02_2.png
Figure 1. Panel A: RFP Fluorescence. Dual-labeled HCT116 cell line expressing tdTomato at 80% cell confluence. Panel B: Nanoluciferase activity. Comparison of luminescence signal for NanoLuc luciferase and negative control demonstrating its robustness and high sensitivity in the Nanoluciferase/tdTomato dual-labeled HCT116 cells. The Nanoluciferase activity was tested 6 hrs after seeding in a 96-well plate. Data are expressed as mean /- SD from duplicates of three independent experiments.
Specifications:
Product Name Nanoluciferase/tdTomato Dual-Reporter HCT116 Cell Line
Shipping Condition Dry Ice - Overnight Shipping
Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Each vial contains about 1 x 106 cells with >95% viability before freezing. Each lot of cells is tested for Nanoluciferase activity, growth and viability following recovery from cryopreservation, and free of mycoplasma and competent lentivirus as well.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.