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Luciferase/tdTomato Dual-Reporter MC38 Cell Line
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Luciferase catalyzes the substrate luciferin in the presence of Mg2+ and ATP, resulting in a flash of light that decays rapidly. This light can be detected by a luminometer. Luciferase from the firefly has become one of the more widely used reporter proteins for both in vitro and in vivo studies, such as, gene transcription activity assay, the noninvasive imaging of reporter gene expression, imaging cerebral strokes, and tracking genetically engineered T cells. Luciferase/tdTomato dual-reporter MC38 (MC38-Luc-RFP) cell line is derived from MC38 cells by transduction of ALSTEM’s pLenti-SFFV-Luciferase-PGK-tdTomator-T2A-PURO Lentiviral Reporter (cat# LR452) lentivirus, which contains Firefly luciferase encoding gene driven by SFFV promoter, followed by tdTomato and puromycin resistance gene by PGK promoter. This cell line stably expresses luciferase at high level. It also expresses a red fluorescent protein (tdTomato) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.
Cell Line: MC38
Reporters: Luciferase, RFP
Selection Marker: Puromycin
Promoter/Transgene: SSFV-Luc-PGK-TdTomato-T2A-puro
Cell Type: Pooled
| Product Name | Luciferase/tdTomato Dual-Reporter MC38 Cell Line |
|---|---|
| Shipping Condition |
Dry Ice - Overnight Shipping |
| Storage and Stability |
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed. |
| Quality Control | Each vial contains about 1 x 106 cells with >95% viability before freezing. Each lot of cells is tested for luciferase activity, growth and viability following recovery from cryopreservation, and free of mycoplasma and competent lentivirus as well. |
| Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
- Nguyen D.H. et al., Reprogramming the tumor immune microenvironment using engineered dual-drug loaded Salmonella. Nat Commun (2024)
- Shimizu D. et al., Pexidartinib and immune checkpoint inhibitors combine to activate tumor immunity in a murine colorectal cancer model by depleting M2 macrophages. Int J Cancer (2024)
- Ariyoshi M. et al., WEE1 inhibitor adavosertib exerts antitumor effects on colorectal cancer, especially in cases with p53 mutations. Cancers (2024)
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