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Lentiviral Reporter Plasmids

Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. Lentiviral vectors packaged as lentiviral particles is one of the most efficient tools to deliver exogenous genes into virtually any types of mammalian cells both in vitro and in vivo, and their use is now commonplace in academic laboratories and industry for both research and clinical gene therapy applications. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into both dividing and non-dividing cells, with low immune response and toxicity in vivo. These viruses also integrate stably into the host genome, enabling long-term transgene expression. Hence, the number of applications to introduce gene product using lentiviruses for in vitro systems, animal models and in clinical trials has been increasing worldwide.

Our third generation lentiviral systems have been designed for increased researcher safety. We have a variety of lentiviral reporter constructs with various promoters, selection and fluorescent markers to visualize and create stable cell lines for your gene of interest. Lentiviral reporter plasmid is an ideal tool for monitoring gene expression or protein trafficking dynamically. Cell line can be engineered by integrating reporter expression cassette (e.g. GFP, RFP and luciferase) into its genomes. With the "visible" tag, these reporter cell lines are valuable tools for various scientific studies, such as analysis of gene expression and regulation, cell labeling and in vivo imaging, tumor evaluation, and drug screening.

Alstem offers four promoters for experimental flexibility in different cell types. Based on our experience and partner feedback, we find the strength of these promoters in many common cell lines, (e.g. HEK293) is SFFV>CAG>CMV>EF1. However, it may vary, as in Jurkat cells, we find EF1>SFFV>CAG~CMV.

Promoter Reporter Selection
EF1 Luciferase Puromycin
CMV NanoLuciferace Neomycin
CAG GFP  
SFFV Luciferase  

Product List

ID Catalog# Name Unit Unit Price (USD) Actions
203 LR152 pLenti-CMV-Luciferase-EF1-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
208 LR242 pLenti-EF1-NanoLuc-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
204 LR153 pLenti-CMV-Luciferase-EF1-GFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
207 LR216 pLenti-EF1-GFP-PGK-Neo Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
202 LR151 pLenti-CMV-Luciferase-EF1-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
199 LR112 pLenti-CMV-GFP-EF1-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
195 LR416 pLenti-SFFV-GFP-PGK-NEO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
196 LR442 pLenti-SFFV-NanoLuc-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
197 LR452 pLenti-SFFV-Luciferase-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
198 LR452F pLenti-SFFV-Luciferase-EF1-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
191 LR353 pLenti-CAG-Luciferase-PGK-GFP-T2A-PURO10 ug $595.00 Add to Cart
192 LR411 pLenti-SFFV-GFP-PGK-Puro Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
193 LR412 pLenti-SFFV-GFP-PGK-RFP-T2A-Puro Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
194 LR415 pLenti-SFFV-GFP-PGK-RFP Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
187 LR315 pLenti-CAG-GFP-PGK-RFP Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
188 LR316 pLenti-CAG-GFP-PGK-NEO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
189 LR342 pLenti-CAG-NanoLuc-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
190 LR352 pLenti-CAG-Luciferase-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
205 LR212 pLenti-EF1-GFP-PGK-RFP-T2A-Puro Lentiviral Reporter Vector10 ug $595.00 Add to Cart
185 LR311 pLenti-CAG-GFP-PGK-Puro Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
186 LR312 pLenti-CAG-GFP-PGK-RFP-T2A-Puro Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
178 LR211 pLenti-EF1-GFP-PGK-Puro Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
206 LR215 pLenti-EF1-GFP-PGK-RFP Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
200 LR115 pLenti-CMV-GFP-EF1-RFP Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
201 LR142 pLenti-CMV-NanoLuc-EF1-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
171 LR110 pLenti-CMV-GFP-EF1-RFP Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
209 LR252 pLenti-EF1-Luciferase-PGK-RFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
210 LR253 pLenti-EF1-Luciferase-PGK-GFP-T2A-PURO Lentiviral Reporter Plasmid10 ug $595.00 Add to Cart
303 LR157 pLenti-CMV-luciferase-EF1-HygR Lentiviral Reporter Plasmid10 μg $595.00 Add to Cart
302 LR356 pLenti-CAG-luciferase-EF1-NeoR Lentiviral Reporter Plasmid10 μg $595.00 Add to Cart
304 LR156 pLenti-CMV-luciferase-EF1-NeoR Lentiviral Reporter Plasmid10 μg $595.00 Add to Cart

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