Products
Gene Editing
Alstem provides CRISPR/Cas9 tools for precise genome editing, targeted gene activation, and targeted gene inhibition. Backed by rigorous validation, our tools empower both fundamental research and translational applications.
CRISPR-Cas9 System
The CRISPR/Cas9 system is a versatile gene-editing tool composed of Cas9 endonuclease and a guide RNA (gRNA). Cas9 acts as molecular scissors, using the gRNA to locate and cleave complementary DNA sequences.
Each gRNA contains a 20-base targeting sequence within a scaffold that binds Cas9 and directs it to the desired genomic site. Cas9 then introduces a double-strand break, which can be repaired by non-homologous end joining (NHEJ), often resulting in small insertions or deletions (indels) that inactivate or disrupt the target gene.
By designing the gRNA sequence, the CRISPR-Cas9 system can be programmed to precisely target virtually any DNA sequence for modification.
