Lentiviral vectors function by integrating transgenes into the host genome, ensuring stable, long-term expression. They efficiently infect both proliferating and quiescent cells with low immunogenicity and minimal toxicity. These reporter plasmids are used together with packaging plasmids and HEK293 cells to generate high-titer lentiviral particles. The viruses are typically pseudotyped with VSV-G, enabling broad cell tropism and efficient transduction across a wide range of mammalian cells. By integrating reporter cassettes (e.g., GFP, RFP, luciferase, NanoLuc) into the genome, researchers can establish stable reporter cell lines for various applications such as cell labeling, in vivo imaging, tumor evaluation, or drug screening.

Our platform uses a third-generation lentiviral system, engineered for enhanced biosafety. Viral functions are separated across multiple helper plasmids to prevent formation of replication-competent viruses. This design allows researchers to generate functional lentiviral particles safely and reliably for both in vitro and preclinical studies.