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All Products Gene Editing Knockout gRNA Vectors

Knockout gRNA Vectors

The CRISPR-Cas9 system has become an increasingly popular gene editing tool, consisting of a Cas9 enzyme and a guide RNA. Cas9 endonuclease acts as molecular scissors and uses CRISPR sequences as guides to recognize and cleave target DNA that contains the complementary sequence. Each guide RNA consists of a pre-designed RNA sequence (about 20 bases long) located within a longer RNA scaffold. The scaffold binds to DNA and the guide RNA ‘guides’ Cas9 to the complementary region in the gene. The guide RNA is responsible for Cas9’s ability to cut at the desired site within the genome. Hence, by manipulating the nucleotide sequence of the guide RNA, the CRISPR-Cas9 system can be programmed to target any DNA sequence for cleavage. The CRISPR-Cas9 technique has been used for a wide variety of applications, such as basic biomedical research, development of biotechnological products, and treatment of medical conditions involving a genetic component.

Alstem offers ready-to-use, gene-specific gRNAs for your gene editing projects. These gRNAs are selected based on their cutting efficiency and specificity by using several online gRNA selection tools. Each selected gRNA is cloned into an expression vector, where a U6 promoter drives the expression of the guide RNA while a CBh promoter controls the expression of an SpCas9 protein and a puromycin resistance gene. These gRNAs have been experimentally validated to ensure their efficient gene editing ability. For a given gene, the gRNAs can be used individually or in pairs to generate frameshift knockouts.  

Product List

ID Catalog# Name Unit Unit Price (USD) Actions
247 G001a CD123-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
248 G001b CD123-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
249 G001c CD123-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
250 G002a FAM171A2-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
251 G002b FAM171A2-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
252 G002c FAM171A2-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
253 G003a FKBP5-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
254 G003b FKBP5-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
255 G003c FKBP5-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
256 G004a LDLR-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
257 G004b LDLR-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
258 G005a LLGL1-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
259 G005b LLGL1-KO gRNA510 μg at 0.5 μg/μL in TE $695.00 Add to Cart
260 G005c LLGL1-KO gRNA610 μg at 0.5 μg/μL in TE $695.00 Add to Cart
261 G005d LLGL1-KO gRNA1410 μg at 0.5 μg/μL in TE $695.00 Add to Cart
262 G006a Neo1-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
263 G006b Neo1-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
264 G006c Neo1-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
265 G007a PLGC2-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
266 G007b PLGC2-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
268 G008a PLN-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
267 G007c PLGC2-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
269 G008b PLN-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
270 G008c PLN-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
271 G009a RABGGTB-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
272 G009b RABGGTB-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
273 G009c RABGGTB-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart
274 G009d RABGGTB-KO gRNA410 μg at 0.5 μg/μL in TE $695.00 Add to Cart
275 G600a Mcu1-KO gRNA110 μg at 0.5 μg/μL in TE $695.00 Add to Cart
276 G600b Mcu1-KO gRNA210 μg at 0.5 μg/μL in TE $695.00 Add to Cart
277 G600c Mcu1-KO gRNA310 μg at 0.5 μg/μL in TE $695.00 Add to Cart

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