Each vector contains a multiple cloning site (MCS) for inserting sequences and drug selection markers before packaging.

For virus production, an expression vector is co-transfected with packaging plasmids into HEK293 cells, the gold-standard producer line. These cells generate high-titer lentiviral particles, which can then be harvested and used to transduce virtually any mammalian cell type, both in vitro and in vivo.

Our third-generation lentiviral system is engineered for maximum biosafety:

• Split-component design: Essential viral genes are separated across multiple plasmids.
• Replication-deficient: Self-replication is eliminated in transduced cells.