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SV40 T Antigen Cell Immortalization Kit

Catalog# Unit Unit Price (USD) Actions
CILV01 5 x 20 ul $1465 Add to Cart
Description:

It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.

Specifications:
Product Name SV40 T Antigen Cell Immortalization Kit
Shipping Condition

Dry Ice - Overnight Shipping

Storage and Stability

Store at -80 °C (stable up to 6 months)

Quality Control

The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization Kit is functionally tested in fibroblasts.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

  1. Benischke AS et al., Sci Rep. 2017 Jul 27;7(1):6656. doi: 10.1038/s41598-017-06523-2.