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Virus Protection Medium

Catalog# Unit Unit Price (USD) Actions
VF010 100 uL $126 Add to Cart
VF050 500 uL $415 Add to Cart
Highlights:
  • Ready-to-use: Simply adding Virus Protection Medium to the virus stock.
  • Long term virus storage: Enable long term virus storage over six months at -80 °C.
  • High recovery efficiency: Preserve 90% of functional titers after six freeze-thaw cycles.
  • Nontoxic: No cytotoxicity was observed.
Description:

Virus Protection Medium can effectively preserve functional viral particles during repetitive freeze-thaw cycles. Usually, lentivirus titer drops up to 50% after one freeze-thaw cycle. By adding Virus Protection Medium to the concentrated virus stock, more than 90% of functional viral particles are preserved, even after six freeze-thaw cycles, which significantly extends the shelf life of the virus stock.

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Figure. GFP lentivirus driven by EF1α promoter was mixed with Virus Protection Medium or control PBS, and multiple aliquots were prepared and treated with different number of freeze-thaw cycles. HEK293T cells were transduced with the lentivirus frozen-thawed aliquots at MOI 0.5 and FACS analysis was performed at 48 hours post-transduction. The percentage of lentivirus titer of aliquots after freeze-thaw cycles was normalized against that of unfrozen aliquots.
images/Desc_Images/VF010_2.png
Figure. GFP lentivirus driven by EF1α promoter was mixed with Virus Protection Medium or control PBS, and multiple aliquots were prepared and treated with different number of freeze-thaw cycles. HEK293T cells were transduced with the lentivirus frozen-thawed aliquots at MOI 0.5 and FACS analysis was performed at 48 hours post-transduction. The percentage of lentivirus titer of aliquots after freeze-thaw cycles was normalized against that of unfrozen aliquots.
Specifications:
Product Name Virus Protection Medium
Shipping Condition Room Temperature - 2 Day Shipping
Storage and Stability This product is stable for 3 months when stored in 4 °C or 6 months if store at -20 °C.
Quality ControlEach lot of Virus Protection Medium is functionally tested in transduction assays using HEK293T cells.
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.