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Human iPS Cell Line (Episomal, CD34+, ApoE Knockout)

Catalog# Unit Unit Price (USD) Actions
iPS36 5x10^5 cells/vial $1890 Add to Cart
Highlights:

ALSTEM’s human isogenic iPS cell lines carrying main APOE variants (APOE-ε2, cat# iPS46; APOE-ε3, cat# iPS26; APOE-ε4, cat# iPS16) and an APOE knock-out line (cat# iPS36) share the same genetic background, and are good candidates for studying AD-related pathologies in neurons induced specifically by ApoE4, as well as for drug discovery. Highlight of these lines include:

  • Derived from one single iPSC clone
  • Sharing the same genetic background
  • Off the shelf - simple thaw the cells and plate them onto serum-free, feeder-free culture
  • Transgene- and virus-free
  • Demonstrated expression of pluripotency markers and endogenous alkaline phosphatase activity
Description:

ALSTEM’s footprint-free human iPS (induced pluripotent stem) cell line with APOE knockout (KO) (cat# iPS36) was derived from the Alstem's iPSC line (cat# iPS16) by advanced genome editing tool. Sequencing results confirmed that this iPS36 isogenic iPSC line has a stable 100 bp homozygous deletion in the exon 2 of APOE gene (Figure 1). The morphology of this human iPS cell line is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and OCT4, and demonstrate strong endogenous alkaline phosphatase activity.

images/Desc_Images/iPS36_1.png
Data Analysis
images/Desc_Images/iPS36_2.png
Figure 1. The sequencing results showed this iPSC line (cat# iPS36) had 100 bp deletion in the exon 2 of APOE gene in both alleles, and Western blotting results confirmed no AproE protein expression in this line.
Specifications:
Product Name Human iPS Cell Line (Episomal, CD34+, ApoE Knockout)
Shipping Condition Dry Ice - Overnight Shipping
Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.