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Human iPS Cells (All-in-one inducible Ngn2)

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Catalog# Unit Unit Price (USD) Actions
iP11NA 5x105 cells/vial $8,900.00 Add to Cart
Highlights:

ALSTEM offers human iPS cell line with inducible expression of Ngn2 by doxycycline (Dox). This cell line is generated from pre-made footprint-free human iPS cells with the inducible Ngn2 that has been stably integrated into the human AAVS1 “Safe Harbor” site. It provides a convenient means to differentiate human iPS cells into excitatory neurons rapidly and efficiently. The integration at “Safe Harbor” sites ensures safe and reliable expression of Ngn2 when Dox is supplied.

  • Highly responsive doxycycline inducible iPS cell line
  • Ideal for in vitro rapid neuronal differentiation
Description:

Neurogenin (Ngn) 2 is a basic helix-loop-helix (bHLH) transcription factor that promotes direct differentiation of pluripotent stem cells to functional excitatory neurons. AAVS1 (also known as PPP1R12C) locus supports robust and sustained transgene expression. Precise insertion of tetracycline inducible Ngn2 into human iPS cells at AAVS1 site could generate a stable iPSC line for rapid neuronal differentiation upon induction.

The ALSTEM scientists developed a highly responsive doxycycline inducible Ngn2-expressing human iPS cell line, which is ideal for in vitro rapid neuronal differentiation. ALSTEM’s doxycycline inducible Ngn2-expressing iPS cell line (cat# iP11NA) is produced from pre-made footprint-free human iPS cells by integrating the all-in-one inducible Ngn2 cassette into the human AAVS1 “Safe Harbor” site.

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This all-in-one cassette consists of both inducible Ngn2 and constitutive M2rtTA that has been inserted into the same allele at the AAVS1 locus by gene editing through HDR pathway, while the other allele of AAVS1 locus remains intact. Upon induction, Ngn2 is expressed and the iPS cells will be directly converted into excitatory neurons.
Specifications:
Product Name Human iPS Cells (All-in-one inducible Ngn2)
Shipping Condition

Dry Ice - Overnight Shipping
Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed
Quality Control

Human iPS cells (inducible Ngn2, neo) were grown in feeder-free conditions with mTeSR1 medium. Each lot of hiPSCs is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.