PLN Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)
|Catalog#||Unit||Unit Price (USD)||Actions|
|iPS41||5x105 cells/vial||$2,835.00||Add to Cart|
ALSTEM offers human iPS cell line (iPS41) with phospholamban (PLN) deficiency This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS11) by using gene editing method to delete whole coding sequence of PLN gene. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 189 bp deletion that contains 159 bp of coding sequence of PLN. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells.
- Good model for studying mechanisms of heart failure associated with PLN
- Ideal for drug discovery when used with PLN wild type iPS cell line (cat# iPS11)
Phospholamban (PLN) is a membrane protein that is regulated by Ca2+-ATPase 2a (SERCA2a), an ATP-driven pump that drives calcium into the cardiac muscle cells. Previous studies show that inhibition of PLN expression and reduction of SERCA2a protein expression results in impaired Ca2+ uptake, which leads to degeneration of cardiomyocytes in heart failure disease state. Additionally, heart failure murine models demonstrate decreased mortality rate with ablation of PLN protein. In addition, ablation of PLN restores the mortality in a heart failure murine model.
ALSTEM’s footprint-free human iPS cell line (cat# iPS41) carries PLN KO mutation in both alleles. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 189 bp deletion including 159 bp of coding sequence of PLN gene. The morphology of this human iPS cell line is identical to that of human ES cells. The cells express the pluripotency markers TRA-1-60, SSEA-3 and OCT4. They also demonstrate a strong endogenous alkaline phosphatase (AP) activity.
|Product Name||PLN Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)|
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|Storage and Stability||
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.