LLGL1 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)
|Catalog#||Unit||Unit Price (USD)||Actions|
|iPS71||5x105 cells/vial||$2,835.00||Add to Cart|
ALSTEM offers human iPS cell line (iPS71) with LLGL1 deficiency. This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS11) by using gene editing method to introduce a deletion in the exon 5 of LLGL1 that resulted in a frame shift mutation. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 100 bp deletion in the exon 5 of LLGL1 gene. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells.
- Ideal for studying Smith-Magenis syndrome and diffuse gastric cancer related pathologies in hepatic ABC transporters and aldosterone synthesis and secretion pathways
- Ideal for drug discovery when used with LLGL1 wild type iPS cell line (cat# iPS11)
Human LLGL1 gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with non-muscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. Mutations of LLGL1 are associated with Smith-Magenis Syndrome and Diffuse Gastric Cancer. Previous studies indicate that LLGL1 is involved in Sertoli-Sertoli Cell Junction Dynamics and PI3K-Akt signaling pathways. Loss of LLGL1 is associated with loss of cellular adhesion and dissemination of cells from colorectal cancer and malignant melanoma, which is significantly correlated with loss of E-cadherin expression.
ALSTEM’s footprint-free human iPS cell line (cat# iPS71) carries LLGL1 KO mutation in both alleles. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 100 bp deletion in the coding region of exon 5 of LLGL1 gene. The morphology of this human iPS cell line is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3, and OCT4. It also demonstrates strong endogenous alkaline phosphatase activity.
|Product Name||LLGL1 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)|
Dry Ice - Overnight Shipping
|Storage and Stability||
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.