pLV-EF1-dCas9-VPR-PGK-BSD
Catalog# | Unit | Unit Price (USD) | Actions |
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DV101 | 10 μg at 0.5 μg/μL in TE | $695.00 | Add to Cart |
Description:
CRISPR (clustered regularly interspaced short palindromic repeats) activation (CRISPRa) is a widely used genetic technique that allows for targeted activation of transcription in bacteria and mammalian cells. The CRISPRa system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional activator such as the tripartite fusion of three transcription activation domains: VP64, p65 and Rta (VPR) and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and recruits transcription factors to increase gene expression, resulting in the activation of the target gene.
pLV-EF1-dCas9-VPR-PGK-BSD Lentiviral Vector expresses the dCas9-VPR fusion with an EF1 promoter and BSD with PGK promoter to allow for selection of transduced cells.
Vector Information
This is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. Ubiquitous EF1 and PGK promoters drive the expression of the dCas9-VPR fusion protein and BSD, respectively, to allow for the selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specific sgRNA to activate the target gene expression.
IMPORTANT NOTICE
Store the vial at -20°C immediately upon receipt.
Specifications:
Product Name | pLV-EF1-dCas9-VPR-PGK-BSD |
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Shipping Condition |
Room Temperature - 2 Day Shipping |
Storage and Stability |
Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed. |
Quality Control | This plasmid is sequence verified. |
Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |