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Gene Activation Vectors

CRISPR (clustered regularly interspaced short palindromic repeats) activation (CRISPRa) is a widely used genetic technique that allows for targeted activation of transcription in bacteria and mammalian cells. The CRISPRa system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional activator such as the tripartite fusion of three transcription activation domains: VP64, p65 and Rta (VPR) and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and recruits transcription factors to increase gene expression, resulting in the activation of the target gene.

Our dCas9-VPR system has two sets of separate lentiviral vectors: the dCas9-VPR vectors and the gRNA expression vectors. There are five individual versions of the dCas9-VPR expression vectors with either an EF1 or an SFFV promoter that drives the dCas9-VPR fusion protein expression and a PGK promoter that controls the expression of a selection marker, either puromycin (PURO) or Blasticidin S (BSD). The gRNA expression vectors consist of three versions with different selection markers BSD, PURO, and NEO (neomycin). Gene specific gRNA that usually targets the promoter region is cloned into one of the gRNA expression vectors. In all the sgRNA vectors, human U6 promoter is used to drive the sgRNA expression.

These two types of lentiviral vectors with different selection markers can be used together by co-transduction into the cells to achieve transcriptional activation of the target gene. Alternatively, a stable dCas9-VPR cell line can be generated and then transduced with a gene-specific gRNA vector to achieve the same purpose.

Product List

ID Catalog# Name Unit Unit Price (USD) Actions
284 DV100 pLV-EF1-dCas9-VPR10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
285 DV101 pLV-EF1-dCas9-VPR-PGK-BSD10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
286 DV102 pLV-EF1-dCas9-VPR-PGK-PURO10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
287 DV201 pLV-SFFV-dCas9-VPR-PGK-BSD10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
288 DV202 pLV-SFFV-dCas9-VPR-PGK-PURO10 μg at 0.5 μg/μL in TE $695.00 Add to Cart

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