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Gene Inhibition Vectors

CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi) is a widely used genetic technique that allows targeted silencing of transcription in bacteria and human cells. The CRISPRi system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional repressor such as the Krüppel-associated box (KRAB) repressor and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and causes a steric block that halts transcript elongation by RNA polymerase, resulting in the repression of the target gene.

Our dCas9-KRAB system has two sets of separate lentiviral vectors: the dCas9-KRAB vectors and the gRNA expression vectors. There are six individual versions of the dCas9-KRAB expression vectors with either an EF1 or a SFFV promoter that drives the dCas9-KRAB fusion protein expression and puromycin-N-acetyltransferase (PURO) or Blasticidin S deaminase (BSD) as the selection marker. The gRNA expression vectors consist of three versions with different selection markers BSD, PURO, and NEO (neomycin). Gene specific gRNA that usually targets the promoter region is cloned into one of the gRNA expression vectors. In all the sgRNA vectors, human U6 promoter is used to drive the sgRNA expression.

These two types of lentivirus vectors with different selection markers can be used together by co-transduction into the cell to achieve transcriptional inhibition of the target gene. Alternatively, a stable dCas9-KRAB cell line can be generated and then transduced with a gene-specific gRNA vector to achieve the same purpose.

Product List

ID Catalog# Name Unit Unit Price (USD) Actions
278 DK111 pLV-EF1-dCas9-KRAB-T2A-BSD10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
279 DK211 pLV-SFFV-dCas9-KRAB-T2A-BSD10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
280 DK110 pLV-EF1-dCas9-KRAB10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
281 DK102 pLV-EF1-dCas9-KRAB-PURO10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
282 DK112 pLV-EF1-dCas9-KRAB-T2A-PURO10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
283 DK202 pLV-SFFV-dCas9-KRAB-PURO10 μg at 0.5 μg/μL in TE $695.00 Add to Cart

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