Description:

CRISPR (clustered regularly interspaced short palindromic repeats) activation (CRISPRa) is a widely used genetic technique that allows for targeted activation of transcription in bacteria and mammalian cells. The CRISPRa system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional activator such as the tripartite fusion of three transcription activation domains: VP64, p65 and Rta (VPR) and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and recruits transcription factors to increase gene expression, resulting in the activation of the target gene.

pLV-SFFV-dCas9-VPR-PGK-BSD Lentiviral Vector expresses the dCas9-VPR fusion with a SFFV promoter and BSD with a PGK promoter to allow for selection of transduced cells.

Vector Information

This is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. In this vector, ubiquitous SFFV and PGK promoters drive the expression of the dCas9-VPR fusion protein and BSD, respectively, to allow for the selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specifc sgRNA to activate the target gene expression.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

Specifications:

Product Name	pLV-SFFV-dCas9-VPR-PGK-BSD
Shipping Condition	Room Temperature - 2 Day Shipping
Storage and Stability	Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control	This plasmid is sequence verified.
Restricted Use	For Research Use Only. Not for use in diagnostic or therapeutic procedures.