Human iPS Cell Line (Episomal, CD34+, ApoE4)
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ALSTEM’s human isogenic iPS cell lines carrying main APOE variants (APOE-ε2, cat# iPS46; APOE-ε3, cat# iPS26; APOE-ε4, cat# iPS16) and an APOE knock-out line (cat# iPS36) share the same genetic background. They are good candidates for studying AD-related pathologies in neurons induced specifically by ApoE4, as well as for drug discovery. Highlight of these lines include:
- Derived from one single iPSC clone
- Sharing the same genetic background
- Off the shelf - simple thaw the cells and plate them onto serum-free, feeder-free culture
- Transgene- and virus-free
- Demonstrated expression of pluripotency markers and endogenous alkaline phosphatase activity
ALSTEM’s footprint-free human iPS (induced pluripotent stem) cell line carried APOE-ε4 in both alleles (cat# iPS16) was derived from human bone marrow CD34+ mononuclear cells by ectopic expression of OCT4, SOX2, KLF4, c-MYC and Lin28 genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and Oct4, and demonstrate strong endogenous alkaline phosphatase activity.
|Human iPS Cell Line (Episomal, CD34+, ApoE4)
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|Storage and Stability
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.