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Human iPS Cell Line (Episomal, CD34+, ApoE4)

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Catalog# Unit Unit Price (USD) Actions
iPS16 5x105 cells/vial $1,999.00 Add to Cart
Highlights:

ALSTEM’s human isogenic iPS cell lines carrying main APOE variants (APOE-ε2, cat# iPS46; APOE-ε3, cat# iPS26; APOE-ε4, cat# iPS16) and an APOE knock-out line (cat# iPS36) share the same genetic background. They are good candidates for studying AD-related pathologies in neurons induced specifically by ApoE4, as well as for drug discovery. Highlight of these lines include:

  • Derived from one single iPSC clone
  • Sharing the same genetic background
  • Off the shelf - simple thaw the cells and plate them onto serum-free, feeder-free culture
  • Transgene- and virus-free
  • Demonstrated expression of pluripotency markers and endogenous alkaline phosphatase activity
Description:

ALSTEM’s footprint-free human iPS (induced pluripotent stem) cell line carried APOE-ε4 in both alleles (cat# iPS16) was derived from human bone marrow CD34+ mononuclear cells by ectopic expression of OCT4, SOX2, KLF4, c-MYC and Lin28 genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells are identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and Oct4, and demonstrate strong endogenous alkaline phosphatase activity.

images/Desc_Images/iPS16_1.png
Figure 1. Characterization of iPSCs derived from human bone marrow CD34 mononuclear cells. Immunostaining of hiPSC colonies expressing ESC specific markers OCT4, Nanog, and TRA-1-60.
images/Desc_Images/iPS16_2.png
Figure 2. The cells have a normal karyotype.
images/Desc_Images/iPS16_3.png
Figure 3. In vivo pluripotency test by teratoma formation. Various cell types such as neural rosette (ectoderm), bone (mesoderm), and glandular structures (endoderm) are found.
Specifications:
Product Name Human iPS Cell Line (Episomal, CD34+, ApoE4)
Shipping Condition

Dry Ice - Overnight Shipping
Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control

Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.