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Luciferase/GFP Dual-Reporter HEK293 Cell Line

Catalog# Unit Unit Price (USD) Actions
LGL01 1 million cells/vial $1,811.00 Add to Cart

Luciferase catalyzes the substrate luciferin in the presence of Mg2+ and ATP, resulting in a flash of light that decays rapidly. This light can be detected by a luminometer. Luciferase from the firefly has become one of the more widely used reporter proteins for both in vitro and in vivo studies, such as, gene transcription activity assay, the noninvasive imaging of reporter gene expression, imaging cerebral strokes, and tracking genetically engineered T cells. Luciferase/GFP dual-reporter HEK293 (HEK293-Luc-GFP) cell line is derived from HEK293 cells by transduction of ALSTEM’s pLenti-CAG-Luciferase-PGK-GFP-T2A-PURO Lentiviral Reporter (cat# LV311G) lentivirus, which contains Firefly luciferase encoding gene driven by CAG promoter, followed by GFP and puromycin resistance gene by PGK promoter. This cell line stably expresses luciferase at high level. It also expresses a green fluorescent protein (GFP) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.

Cell Line: HEK293

Reporters: Luciferase, GFP

Selection Marker: Puromycin

Promoter/Transgene: CAG-Luc-PGK-GFP-T2A-puro

Cell Type: Single Clone

Figure 1. Panel A: GFP Fluorescence. Dual-labeled HEK293 cell line expressing GFP at 90% cell confluence. Panel B: Luciferase activity. Dual-labeled HEK293 cells were plated into a 96-well plate. The luciferase activity was tested 6 hrs after seeding. Data are expressed as mean /- SD from duplicates of three independent experiments.
Figure 2. The lentiviral reporter vector contains luciferase gene driven by CAG promoter, GFP and puromycin resistance gene by PGK promoter.
Product Name Luciferase/GFP Dual-Reporter HEK293 Cell Line
Shipping Condition

Dry Ice - Overnight Shipping

Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Each vial contains about 1 x 106 cells with >95% viability before freezing. Each lot of cells is tested for luciferase activity, growth and viability following recovery from cryopreservation, and free of mycoplasma and competent lentivirus as well.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.