• Validated and well-characterized
• Straightforward and easy workflow
• Precise integration of any gene at AAVS1 safe harbor site
• Distinctive design ensures robust and consistent transgene expression
• Minimal off-target integration risk
• Efficient process for CRISPR-Cas9 library screening
• A versatile combination approach provides the most extensive options for CRISPR-Cas9-AAVS1 Safe Harbor Site Targeting System

One of the most powerful applications of genome editing with the CRISPR/Cas9 system is the introduction of precise modifications at a targeted location, which exploits the homology-directed repair (HDR) pathway in mammalian cells. An important application involves knocking in an expression cassette into the AAVS1 safe-harbor site (PPP1R12C). This technique allows the introduction of a transgene for overexpression and rescue studies without disrupting the expression of endogenous genes. Such studies not only provide insights into disease mechanisms but also offer potential therapeutic treatments for various types of disease.

ALSTEM has developed its CRISPR/Cas9 based AAVS1 Safe Harbor Site Targeting System for safe and easy gene knock-in. We offer an array of tools designed to help researchers accelerating their research by leveraging the AAVS1 safe harbor site. Click here for more information.