SV40 T Antigen Cell Immortalization Kit
It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.
|Product Name||SV40 T Antigen Cell Immortalization Kit|
Dry Ice - Overnight Shipping
|Storage and Stability||
Store at -80 °C (stable up to 6 months)
The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization Kit is functionally tested in fibroblasts.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Benischke AS et al., Sci Rep. 2017 Jul 27;7(1):6656. doi: 10.1038/s41598-017-06523-2.