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Human iPS Cells (inducible Ngn2)

Catalog# Unit Unit Price (USD) Actions
iP11N 5x10^5 cells/vial $5000 Add to Cart
Highlights:

ALSTEM offers human iPS cell line with inducible expression of Ngn2 by doxycycline (Dox). This cell line is generated from pre-made in footprint-free human iPS cells with the inducible Ngn2 that has been stably integrated into the human AAVS1 “Safe Harbor” site. It provides a convenient means to differentiate human iPS cells to excitatory neurons rapidly and efficiently. The integration at “Safe Harbor” sites ensures safe and reliable expression of Ngn2 when Dox is supplied.

  • Highly responsive doxycycline inducible iPS cell line
  • Ideal for in vitro rapid neuronal differentiation
Description:

Neurogenin (Ngn) 2 is a basic helix-loop-helix (bHLHtranscription factor that promotes direct differentiation of pluripotent stem cells to functional excitatory neurons. AAVS1 (also known as PPP1R12C) locus supports robust and sustained transgene expression. Precise insertion of tetracycline inducible Ngn2 into human iPS cells at AAVS1 site could generate a stable iPSC line for rapid neuronal differentiation upon induction.

 The ALSTEM scientists developed a highly responsive doxycycline inducible Ngn2-expressing human iPS cell line, ideal for in vitro rapid neuronal differentiation. In the absence of doxycycline (Dox), the cells retain the pluripotent state; however, upon induction by Dox, the cells will differentiate into functional excitatory neurons rapidly.   

ALSTEM’s doxycycline inducible Ngn2-expressing cell line (cat# iP11N) is available pre-made in footprint-free human iPS cells with the inducible Ngn2 stably integrated into the human AAVS1 “Safe Harbor” site. Upon induction by doxycycline (Dox), these iPS cells will differentiate into excitatory neurons in one week.

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Ngn2 and M2rtTA will be inserted at the AAVS1 locus through HDR pathway. Upon induction, the iPS cells will be directly converted into excitatory neurons upon the expression of Ngn2. 
Specifications:
Product Name Human iPS Cells (inducible Ngn2)
Shipping Condition

Dry ice - Overnight

Storage and Stability

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Human iPS cells (inducible Ngn2) were grown in feeder-free conditions with mTeSR1 medium. Each lot of hiPSCs is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.